dylight 594 conjugated uea1  (Vector Laboratories)

Journal: The American Journal of Pathology

Article Title: Neonatal Thymic Dynamics Influence Autoimmune Pathology by Shaping the Suppressive Potential of Regulatory T Cells

doi: 10.1016/j.ajpath.2025.12.007

Figure Lengend Snippet: Thymic epithelial cell (TEC) subpopulations in the neonatal thymus. A: Representative flow cytometry profiles of CD45 − epithelial cell adhesion molecule (EpCAM) + TECs at postnatal days 1, 3, 5, and 7. B: Total number of TECs in the thymus from the indicated time points. C: Representative flow cytometry profiles of medullary TECs [mTECs; ulex europaeus agglutinin 1 (UEA1) + Ly51 – ] and cortical TECs (cTECs; UEA1 – Ly51 + ) within the CD45 − EpCAM + TEC population from the indicated time points. D: Total number of mTECs ( left panel ) and cTECs ( right panel ) in the thymus from the indicated mice. E: Representative flow cytometry profiles of autoimmune regulator (Aire) expression in mTECs from the indicated time points. F: Frequency ( left panel ) and absolute number ( right panel ) of Aire + mTECs. At least three independent experiments were conducted using neonatal mice. Data are presented as means ± SEM ( B , D , and F ). n = 4 or 5 mice per group ( B , D , and F ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001. Max, maximum; NS, not significant.

Article Snippet: Isolated TECs were stained with the following antibodies: BV421-conjugated anti-mouse CD45 (BD Biosciences, Franklin Lakes, NJ; 30-F11); phosphatidylethanolamine-Cy7–conjugated anti-mouse epithelial cell adhesion molecule (BioLegend; G8.8); Alexa Fluor 647–conjugated anti-mouse Ly-51 (BioLegend; 6C3); DyLight 594–conjugated ulex europaeus agglutinin 1 (UEA1) (Vector Laboratories, Newark, CA; DL-1067-1); and Alexa Fluor 488–conjugated anti-mouse Aire (eBioscience; 5H12).

Techniques: Flow Cytometry, Expressing

Journal: Cell host & microbe

Article Title: HMGB1 functions as a critical mediator of host defense at the gut mucosal barrier

doi: 10.1016/j.chom.2026.01.008

Figure Lengend Snippet: (A) Immunofluorescent (IF) staining for HMGB1 (yellow) and Hoechst (blue) in human colon. n = 16. (B) Quantification of surface HMGB1 in (A). Staining intensity as relative fluorescent units (RFU) per μm 2 . (C) Surface HMGB1 in (B) graphed by inflammation severity. (D) IF for HMGB1 (yellow), Ulex europaeus agglutinin-1 (UEA-1) (magenta), and Hoechst (blue) in whole-mouse colon. Scale bars, 2 mm and 50 μm. n = 6. (E) IF for HMGB1 (yellow), UEA-1 (magenta), and Hoechst (blue) in mouse colon. n = 6. (F) IF for HMGB1 (yellow) and Hoechst (blue) in proximal mouse colon. Arrows indicate epithelial surface. n = 20. (G) HMGB1 concentration in murine colonic mucus measured by ELISA. n = 6. (H) Immunoblotting (IB) for HMGB1 in murine colonic mucus. n = 7. (I) IF for HMGB1 (yellow) and Hoechst (blue) in proximal mouse colon. Arrows indicate epithelial surface. n = 6. (J) IB for HMGB1 in epithelial scrapings from mouse colon. n = 4. (K) HMGB1 concentration in mouse stool by ELISA. n = 6. All data are reported as mean ± s.d. and each datapoint represents one individual biological replicate. Scale bars, 100 μm and original magnification is 400x, unless otherwise noted.

Article Snippet: Ulex europaeus agglutinin I (UEA-1) , Vector Laboratories , DL-1067-1.

Techniques: Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot